Transcriptome analysis reveals novel insights into the response to Pb exposure in Phanerochaete chrysosporium.
Identifieur interne : 000096 ( Main/Exploration ); précédent : 000095; suivant : 000097Transcriptome analysis reveals novel insights into the response to Pb exposure in Phanerochaete chrysosporium.
Auteurs : Chao Huang [République populaire de Chine] ; Rongzhong Wang [République populaire de Chine] ; Guangming Zeng [République populaire de Chine] ; Danlian Huang [République populaire de Chine] ; Cui Lai [République populaire de Chine] ; Jiachao Zhang [République populaire de Chine] ; Zhihua Xiao [République populaire de Chine] ; Jia Wan [République populaire de Chine] ; Piao Xu [République populaire de Chine] ; Xiaomin Gong [République populaire de Chine] ; Wenjing Xue [République populaire de Chine] ; Xiaoya Ren [République populaire de Chine]Source :
- Chemosphere [ 1879-1298 ] ; 2018.
Descripteurs français
- KwdFr :
- Analyse de polymorphisme de longueur de fragments amplifiés (MeSH), Analyse de profil d'expression de gènes (MeSH), Gènes fongiques (effets des médicaments et des substances chimiques), Métaux lourds (pharmacologie), Phanerochaete (effets des médicaments et des substances chimiques), Plomb (pharmacologie), Transcription génétique (effets des médicaments et des substances chimiques).
- MESH :
- effets des médicaments et des substances chimiques : Gènes fongiques, Phanerochaete, Transcription génétique.
- pharmacologie : Métaux lourds, Plomb.
- Analyse de polymorphisme de longueur de fragments amplifiés, Analyse de profil d'expression de gènes.
English descriptors
- KwdEn :
- MESH :
- chemical , pharmacology : Lead, Metals, Heavy.
- drug effects : Genes, Fungal, Phanerochaete, Transcription, Genetic.
- Amplified Fragment Length Polymorphism Analysis, Gene Expression Profiling.
Abstract
Metals released into the environment continue to be of concern for human health. Using white-rot fungi as biosorbents for heavy metals removal is an attractive alternative owing to its good performance and low cost. However, the molecular mechanism underlying heavy metal tolerance in white-rot fungi has not yet been fully elucidated. This study identified and analyzed the lead (Pb)-induced transcriptional changes in Phanerochaete chrysosporium, a well-known heavy metal hyperaccumulating white-rot fungus. The results confirmed its outstanding ability in Pb tolerance and effective defense system. By comparative analysis of gene expression profiles obtained from cDNA-amplified fragment length polymorphism (cDNA-AFLP), we isolated 43 transcript-derived fragments (TDFs) differentially regulated by Pb exposure in P. chrysosporium, and 23 TDFs presented significant similarities to genes encoding known or putative proteins which belong to different functional categories involving ion binding, energy and carbohydrate metabolism, and signal transduction. The detailed characterization of these Pb-responsive genes was presented and the expression patterns of some interesting genes were validated by quantitative RT-PCR. This work provides the first evidence of Pb-responsive genes along with their putatively functional annotations in P. chrysosporium, which may help to understand the mechanism underlying heavy metal accumulation and tolerance in P. chrysosporium.
DOI: 10.1016/j.chemosphere.2017.12.046
PubMed: 29245132
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Transcriptome analysis reveals novel insights into the response to Pb exposure in Phanerochaete chrysosporium.</title><author><name sortKey="Huang, Chao" sort="Huang, Chao" uniqKey="Huang C" first="Chao" last="Huang">Chao Huang</name><affiliation wicri:level="1"><nlm:affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082</wicri:regionArea><wicri:noRegion>Changsha 410082</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Rongzhong" sort="Wang, Rongzhong" uniqKey="Wang R" first="Rongzhong" last="Wang">Rongzhong Wang</name><affiliation wicri:level="1"><nlm:affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082</wicri:regionArea><wicri:noRegion>Changsha 410082</wicri:noRegion></affiliation></author><author><name sortKey="Zeng, Guangming" sort="Zeng, Guangming" uniqKey="Zeng G" first="Guangming" last="Zeng">Guangming Zeng</name><affiliation wicri:level="1"><nlm:affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China. Electronic address: zgming@hnu.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082</wicri:regionArea><wicri:noRegion>Changsha 410082</wicri:noRegion></affiliation></author><author><name sortKey="Huang, Danlian" sort="Huang, Danlian" uniqKey="Huang D" first="Danlian" last="Huang">Danlian Huang</name><affiliation wicri:level="1"><nlm:affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China. Electronic address: huangdanlian@hnu.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082</wicri:regionArea><wicri:noRegion>Changsha 410082</wicri:noRegion></affiliation></author><author><name sortKey="Lai, Cui" sort="Lai, Cui" uniqKey="Lai C" first="Cui" last="Lai">Cui Lai</name><affiliation wicri:level="1"><nlm:affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082</wicri:regionArea><wicri:noRegion>Changsha 410082</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Jiachao" sort="Zhang, Jiachao" uniqKey="Zhang J" first="Jiachao" last="Zhang">Jiachao Zhang</name><affiliation wicri:level="1"><nlm:affiliation>College of Resources and Environment, Hunan Agricultural University, Changsha 410128, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Resources and Environment, Hunan Agricultural University, Changsha 410128</wicri:regionArea><wicri:noRegion>Changsha 410128</wicri:noRegion></affiliation></author><author><name sortKey="Xiao, Zhihua" sort="Xiao, Zhihua" uniqKey="Xiao Z" first="Zhihua" last="Xiao">Zhihua Xiao</name><affiliation wicri:level="1"><nlm:affiliation>College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128</wicri:regionArea><wicri:noRegion>Changsha 410128</wicri:noRegion></affiliation></author><author><name sortKey="Wan, Jia" sort="Wan, Jia" uniqKey="Wan J" first="Jia" last="Wan">Jia Wan</name><affiliation wicri:level="1"><nlm:affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082</wicri:regionArea><wicri:noRegion>Changsha 410082</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Piao" sort="Xu, Piao" uniqKey="Xu P" first="Piao" last="Xu">Piao Xu</name><affiliation wicri:level="1"><nlm:affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082</wicri:regionArea><wicri:noRegion>Changsha 410082</wicri:noRegion></affiliation></author><author><name sortKey="Gong, Xiaomin" sort="Gong, Xiaomin" uniqKey="Gong X" first="Xiaomin" last="Gong">Xiaomin Gong</name><affiliation wicri:level="1"><nlm:affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082</wicri:regionArea><wicri:noRegion>Changsha 410082</wicri:noRegion></affiliation></author><author><name sortKey="Xue, Wenjing" sort="Xue, Wenjing" uniqKey="Xue W" first="Wenjing" last="Xue">Wenjing Xue</name><affiliation wicri:level="1"><nlm:affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082</wicri:regionArea><wicri:noRegion>Changsha 410082</wicri:noRegion></affiliation></author><author><name sortKey="Ren, Xiaoya" sort="Ren, Xiaoya" uniqKey="Ren X" first="Xiaoya" last="Ren">Xiaoya Ren</name><affiliation wicri:level="1"><nlm:affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082</wicri:regionArea><wicri:noRegion>Changsha 410082</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29245132</idno><idno type="pmid">29245132</idno><idno type="doi">10.1016/j.chemosphere.2017.12.046</idno><idno type="wicri:Area/Main/Corpus">000135</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000135</idno><idno type="wicri:Area/Main/Curation">000135</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000135</idno><idno type="wicri:Area/Main/Exploration">000135</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Transcriptome analysis reveals novel insights into the response to Pb exposure in Phanerochaete chrysosporium.</title><author><name sortKey="Huang, Chao" sort="Huang, Chao" uniqKey="Huang C" first="Chao" last="Huang">Chao Huang</name><affiliation wicri:level="1"><nlm:affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082</wicri:regionArea><wicri:noRegion>Changsha 410082</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Rongzhong" sort="Wang, Rongzhong" uniqKey="Wang R" first="Rongzhong" last="Wang">Rongzhong Wang</name><affiliation wicri:level="1"><nlm:affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082</wicri:regionArea><wicri:noRegion>Changsha 410082</wicri:noRegion></affiliation></author><author><name sortKey="Zeng, Guangming" sort="Zeng, Guangming" uniqKey="Zeng G" first="Guangming" last="Zeng">Guangming Zeng</name><affiliation wicri:level="1"><nlm:affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China. Electronic address: zgming@hnu.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082</wicri:regionArea><wicri:noRegion>Changsha 410082</wicri:noRegion></affiliation></author><author><name sortKey="Huang, Danlian" sort="Huang, Danlian" uniqKey="Huang D" first="Danlian" last="Huang">Danlian Huang</name><affiliation wicri:level="1"><nlm:affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China. Electronic address: huangdanlian@hnu.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082</wicri:regionArea><wicri:noRegion>Changsha 410082</wicri:noRegion></affiliation></author><author><name sortKey="Lai, Cui" sort="Lai, Cui" uniqKey="Lai C" first="Cui" last="Lai">Cui Lai</name><affiliation wicri:level="1"><nlm:affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082</wicri:regionArea><wicri:noRegion>Changsha 410082</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Jiachao" sort="Zhang, Jiachao" uniqKey="Zhang J" first="Jiachao" last="Zhang">Jiachao Zhang</name><affiliation wicri:level="1"><nlm:affiliation>College of Resources and Environment, Hunan Agricultural University, Changsha 410128, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Resources and Environment, Hunan Agricultural University, Changsha 410128</wicri:regionArea><wicri:noRegion>Changsha 410128</wicri:noRegion></affiliation></author><author><name sortKey="Xiao, Zhihua" sort="Xiao, Zhihua" uniqKey="Xiao Z" first="Zhihua" last="Xiao">Zhihua Xiao</name><affiliation wicri:level="1"><nlm:affiliation>College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128</wicri:regionArea><wicri:noRegion>Changsha 410128</wicri:noRegion></affiliation></author><author><name sortKey="Wan, Jia" sort="Wan, Jia" uniqKey="Wan J" first="Jia" last="Wan">Jia Wan</name><affiliation wicri:level="1"><nlm:affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082</wicri:regionArea><wicri:noRegion>Changsha 410082</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Piao" sort="Xu, Piao" uniqKey="Xu P" first="Piao" last="Xu">Piao Xu</name><affiliation wicri:level="1"><nlm:affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082</wicri:regionArea><wicri:noRegion>Changsha 410082</wicri:noRegion></affiliation></author><author><name sortKey="Gong, Xiaomin" sort="Gong, Xiaomin" uniqKey="Gong X" first="Xiaomin" last="Gong">Xiaomin Gong</name><affiliation wicri:level="1"><nlm:affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082</wicri:regionArea><wicri:noRegion>Changsha 410082</wicri:noRegion></affiliation></author><author><name sortKey="Xue, Wenjing" sort="Xue, Wenjing" uniqKey="Xue W" first="Wenjing" last="Xue">Wenjing Xue</name><affiliation wicri:level="1"><nlm:affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082</wicri:regionArea><wicri:noRegion>Changsha 410082</wicri:noRegion></affiliation></author><author><name sortKey="Ren, Xiaoya" sort="Ren, Xiaoya" uniqKey="Ren X" first="Xiaoya" last="Ren">Xiaoya Ren</name><affiliation wicri:level="1"><nlm:affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082</wicri:regionArea><wicri:noRegion>Changsha 410082</wicri:noRegion></affiliation></author></analytic><series><title level="j">Chemosphere</title><idno type="eISSN">1879-1298</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amplified Fragment Length Polymorphism Analysis (MeSH)</term><term>Gene Expression Profiling (MeSH)</term><term>Genes, Fungal (drug effects)</term><term>Lead (pharmacology)</term><term>Metals, Heavy (pharmacology)</term><term>Phanerochaete (drug effects)</term><term>Transcription, Genetic (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de polymorphisme de longueur de fragments amplifiés (MeSH)</term><term>Analyse de profil d'expression de gènes (MeSH)</term><term>Gènes fongiques (effets des médicaments et des substances chimiques)</term><term>Métaux lourds (pharmacologie)</term><term>Phanerochaete (effets des médicaments et des substances chimiques)</term><term>Plomb (pharmacologie)</term><term>Transcription génétique (effets des médicaments et des substances chimiques)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Lead</term><term>Metals, Heavy</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Genes, Fungal</term><term>Phanerochaete</term><term>Transcription, Genetic</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Gènes fongiques</term><term>Phanerochaete</term><term>Transcription génétique</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Métaux lourds</term><term>Plomb</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amplified Fragment Length Polymorphism Analysis</term><term>Gene Expression Profiling</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de polymorphisme de longueur de fragments amplifiés</term><term>Analyse de profil d'expression de gènes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Metals released into the environment continue to be of concern for human health. Using white-rot fungi as biosorbents for heavy metals removal is an attractive alternative owing to its good performance and low cost. However, the molecular mechanism underlying heavy metal tolerance in white-rot fungi has not yet been fully elucidated. This study identified and analyzed the lead (Pb)-induced transcriptional changes in Phanerochaete chrysosporium, a well-known heavy metal hyperaccumulating white-rot fungus. The results confirmed its outstanding ability in Pb tolerance and effective defense system. By comparative analysis of gene expression profiles obtained from cDNA-amplified fragment length polymorphism (cDNA-AFLP), we isolated 43 transcript-derived fragments (TDFs) differentially regulated by Pb exposure in P. chrysosporium, and 23 TDFs presented significant similarities to genes encoding known or putative proteins which belong to different functional categories involving ion binding, energy and carbohydrate metabolism, and signal transduction. The detailed characterization of these Pb-responsive genes was presented and the expression patterns of some interesting genes were validated by quantitative RT-PCR. This work provides the first evidence of Pb-responsive genes along with their putatively functional annotations in P. chrysosporium, which may help to understand the mechanism underlying heavy metal accumulation and tolerance in P. chrysosporium.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">29245132</PMID><DateCompleted><Year>2018</Year><Month>04</Month><Day>02</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-1298</ISSN><JournalIssue CitedMedium="Internet"><Volume>194</Volume><PubDate><Year>2018</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Chemosphere</Title><ISOAbbreviation>Chemosphere</ISOAbbreviation></Journal><ArticleTitle>Transcriptome analysis reveals novel insights into the response to Pb exposure in Phanerochaete chrysosporium.</ArticleTitle><Pagination><MedlinePgn>657-665</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0045-6535(17)32016-7</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.chemosphere.2017.12.046</ELocationID><Abstract><AbstractText>Metals released into the environment continue to be of concern for human health. Using white-rot fungi as biosorbents for heavy metals removal is an attractive alternative owing to its good performance and low cost. However, the molecular mechanism underlying heavy metal tolerance in white-rot fungi has not yet been fully elucidated. This study identified and analyzed the lead (Pb)-induced transcriptional changes in Phanerochaete chrysosporium, a well-known heavy metal hyperaccumulating white-rot fungus. The results confirmed its outstanding ability in Pb tolerance and effective defense system. By comparative analysis of gene expression profiles obtained from cDNA-amplified fragment length polymorphism (cDNA-AFLP), we isolated 43 transcript-derived fragments (TDFs) differentially regulated by Pb exposure in P. chrysosporium, and 23 TDFs presented significant similarities to genes encoding known or putative proteins which belong to different functional categories involving ion binding, energy and carbohydrate metabolism, and signal transduction. The detailed characterization of these Pb-responsive genes was presented and the expression patterns of some interesting genes were validated by quantitative RT-PCR. This work provides the first evidence of Pb-responsive genes along with their putatively functional annotations in P. chrysosporium, which may help to understand the mechanism underlying heavy metal accumulation and tolerance in P. chrysosporium.</AbstractText><CopyrightInformation>Copyright © 2017 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Chao</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Rongzhong</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zeng</LastName><ForeName>Guangming</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China. Electronic address: zgming@hnu.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Danlian</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China. Electronic address: huangdanlian@hnu.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lai</LastName><ForeName>Cui</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Jiachao</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>College of Resources and Environment, Hunan Agricultural University, Changsha 410128, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xiao</LastName><ForeName>Zhihua</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wan</LastName><ForeName>Jia</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Piao</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gong</LastName><ForeName>Xiaomin</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xue</LastName><ForeName>Wenjing</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ren</LastName><ForeName>Xiaoya</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>College of Environmental Science and Engineering, Hunan University, Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>12</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Chemosphere</MedlineTA><NlmUniqueID>0320657</NlmUniqueID><ISSNLinking>0045-6535</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019216">Metals, Heavy</NameOfSubstance></Chemical><Chemical><RegistryNumber>2P299V784P</RegistryNumber><NameOfSubstance UI="D007854">Lead</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D054458" MajorTopicYN="N">Amplified Fragment Length Polymorphism Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="Y">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005800" MajorTopicYN="N">Genes, Fungal</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007854" MajorTopicYN="N">Lead</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019216" MajorTopicYN="N">Metals, Heavy</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014158" MajorTopicYN="N">Transcription, Genetic</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Gene expression</Keyword><Keyword MajorTopicYN="N">Pb</Keyword><Keyword MajorTopicYN="N">Phanerochaete chrysosporium</Keyword><Keyword MajorTopicYN="N">TDFs</Keyword><Keyword MajorTopicYN="N">cDNA-AFLP</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>10</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2017</Year><Month>11</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>12</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>12</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>4</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>12</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29245132</ArticleId><ArticleId IdType="pii">S0045-6535(17)32016-7</ArticleId><ArticleId IdType="doi">10.1016/j.chemosphere.2017.12.046</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Huang, Chao" sort="Huang, Chao" uniqKey="Huang C" first="Chao" last="Huang">Chao Huang</name></noRegion><name sortKey="Gong, Xiaomin" sort="Gong, Xiaomin" uniqKey="Gong X" first="Xiaomin" last="Gong">Xiaomin Gong</name><name sortKey="Huang, Danlian" sort="Huang, Danlian" uniqKey="Huang D" first="Danlian" last="Huang">Danlian Huang</name><name sortKey="Lai, Cui" sort="Lai, Cui" uniqKey="Lai C" first="Cui" last="Lai">Cui Lai</name><name sortKey="Ren, Xiaoya" sort="Ren, Xiaoya" uniqKey="Ren X" first="Xiaoya" last="Ren">Xiaoya Ren</name><name sortKey="Wan, Jia" sort="Wan, Jia" uniqKey="Wan J" first="Jia" last="Wan">Jia Wan</name><name sortKey="Wang, Rongzhong" sort="Wang, Rongzhong" uniqKey="Wang R" first="Rongzhong" last="Wang">Rongzhong Wang</name><name sortKey="Xiao, Zhihua" sort="Xiao, Zhihua" uniqKey="Xiao Z" first="Zhihua" last="Xiao">Zhihua Xiao</name><name sortKey="Xu, Piao" sort="Xu, Piao" uniqKey="Xu P" first="Piao" last="Xu">Piao Xu</name><name sortKey="Xue, Wenjing" sort="Xue, Wenjing" uniqKey="Xue W" first="Wenjing" last="Xue">Wenjing Xue</name><name sortKey="Zeng, Guangming" sort="Zeng, Guangming" uniqKey="Zeng G" first="Guangming" last="Zeng">Guangming Zeng</name><name sortKey="Zhang, Jiachao" sort="Zhang, Jiachao" uniqKey="Zhang J" first="Jiachao" last="Zhang">Jiachao Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhanerochaeteV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000096 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000096 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhanerochaeteV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:29245132
|texte= Transcriptome analysis reveals novel insights into the response to Pb exposure in Phanerochaete chrysosporium.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:29245132" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhanerochaeteV1
| This area was generated with Dilib version V0.6.37. |  |